If referring to the fluid within the pressure vessel itself, generally, no.
However, fluid within the piping will affect the loads on the nozzles, and thus should be included within the analysis.
Further, if you are performing a full analysis of the nozzle, the design pressure of the vessel should have any hydrostatic head built into it.
Note that this applies to any equipment whose nozzle does not move as a function of fluid density or level. Large tanks do not meet this requirement.